US 8,138,309 B2 · Modified rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1,5-bisphosphate

Abstract

The present invention generally relates to a modified Rubisco large subunit εN-Methyltransferase (Rubisco LSMT, or RLSMT). The present invention also relates to a modified RLSMT-carbonic anhydrase (RLSMT-CA). This modified RLSMT-CA improves the efficiency of the reduction of CO2 during photosynthesis, which may increase plant growth rates. The present invention also relates to nucleic acids encoding the modified RLSMT-CA or modified RLSMT. Also, the present invention relates to cells including the modified RLSMT-CA or modified RLSMT, plants containing the modified RLSMT-CA or modified RLSMT, and methods using compositions of the present invention. In addition, the present invention relates to antibodies conjugated to CA which may bind to Rubisco, and antibodies which bind a modified RLSMT-CA. The invention also relates to modified forms of the LS and SS of Rubisco where the modified forms are fusions with CA or biologically active fragments thereof. The present invention provides methods of altering Rubisco carboxylase activity and altering plant growth.

Claims14

Dependent claims

A modified Rubisco large subunit εN-methyltransferase (RLSMT), comprising a Rubisco large subunit εN-methyltransferase linked to a second molecule and wherein the modified RLSMT has the ability to bind a Rubisco.

Dependencies are inferred from the claim text.

Facts

Application

12/190,246Filed 12 Aug 2008

Published

US 2009/0070901 A112 Mar 2009

Granted

20 Mar 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases
C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
Y02A 40/146
In agriculture: genetically Modified [GMO] plants, e.g. transgenic plants
Y02P 60/20
Technologies relating to agriculture, livestock or agroalimentary industries: reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2

Modified rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1,5-bisphosphate

Life of this patent

Modified rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1,5-bisphosphate

Life of this patent

Abstract

Claims14