Reagents, methods and systems to suppress pro-inflammatory cytokines
AssigneeWarsaw Orthopedic, Inc.
InventorJeffrey C. Marx
Life of this patent
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Abstract
The present invention relates to reagents, methods and systems to treat inflammation and pain in a subject using small interfering RNA (siRNA) molecules targeted to either TNFα, IL1, IL6 and other pro-inflammatory cytokines.
Claims17
A depot implant comprising:
- a body;
- and
- a siRNA molecule corresponding to at least a portion of a pro-inflammatory cytokine nucleic acid sequence capable of inhibiting expression of a pro-inflammatory cytokine in a cell, wherein the siRNA molecule is disposed in the body and the body is capable of eluting the siRNA molecule,
- wherein the siRNA molecule comprises a sense strand and the sense strand comprises a sequence that is the same as SEQ ID NO:
- 7.
A depot implant comprising:
- a body;
- and
- an expression vector comprising at least one DNA sequence encoding a siRNA molecule that comprises a sense strand that comprises a sequence that is the same as SEQ ID NO:
- 7 corresponding to at least a portion of a pro-inflammatory cytokine nucleic acid sequence capable of inhibiting expression of pro-inflammatory cytokine in a cell operably linked to a genetic control element capable of directing expression of said siRNA molecule in a host cell, wherein the expression vector is disposed in the body
- and the body is capable of eluting the expression vector.
A system for treating a patient suffering from inflammation, the system comprising at least one siRNA molecule that corresponds to at least a portion of a pro-inflammatory cytokine nucleic acid sequence and a means for introducing said siRNA molecule to a cell of the patient, wherein the siRNA comprises a sense strand that comprises a sequence that is the same as SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.