Method for removing endotoxin from proteins
AssigneeGlanbia Nutritionals (Ireland) Ltd.
InventorsKevin Thomson, Loren S. Ward, Stanley Wrobel
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Abstract
Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.
Claims8
A method for treating a biofilm-associated disease condition in a human or animal, the method comprising administering to the human or animal a therapeutically effective amount of an endotoxin-free bovine lactoferrin product made by removing bound endotoxin from lactoferrin by a method comprising the steps of
- a) binding the lactoferrin to a cation exchange resin;
- b) eluting the endotoxin using a low-ionic strength solution in the absence of added surfactant;
- and
- c) eluting the lactoferrin with a high ionic strength solution.
A method for improving healing in an acute or chronic wound, the method comprising administering to the human or animal a therapeutically effective amount of an endotoxin-free bovine lactoferrin product made by removing bound endotoxin from lactoferrin by a method comprising the steps of
- a) binding the lactoferrin to a cation exchange resin;
- b) eluting the endotoxin using a low-ionic strength solution in the absence of added surfactant;
- and
- c) eluting the lactoferrin with a high ionic strength solution.
Dependencies are inferred from the claim text.