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AssigneeFuji Film Manufacturing Europe B.V.
InventorsJan Bastiaan Bouwstra, Andries Johannes Jozef Van Es, Yuzo Toda
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The invention concerns a cell support comprising an RGD-enriched gelatine that has a more even distribution of RGD sequences than occurring in a natural gelatine and with a minimum level of RGD sequences. More precise the percentage of RGD sequences related to the total number of amino acids is at least 0.4 and if the RGD-enriched gelatine comprises 350 amino acids or more, each stretch of 350 amino acids contains at least one RGD motif. Preferably the RGD-enriched gelatines are prepared by recombinant technology, and have a sequence that is derived from a human gelatine or collagen amino acid sequence. The invention also relates to RGD-enriched gelatines that are used for attachment to integrins. In particular The RGD-enriched gelatines of the invention are suitable for coating a cell culture support for growing anchor-dependant cell types. Further, the RGD-enriched gelatines of the invention may find use in medical applications, in particular as a coating on implant or transplant material or as a component of drug delivery systems.
A method for inhibition of cancer metastasis in a subject in need thereof, said method comprising administering to the subject an RGD-enriched gelatine comprising at least 4 RGD motifs, and having a percentage of RGD motifs relative to the total number of amino acids in the RGD-enriched gelatine of at least 1.5, wherein if the RGD-enriched gelatine comprises 350 amino acids or more, each stretch of 350 amino acids contains at least one RGD motif, and wherein the cancer metastasis is inhibited.
Dependencies are inferred from the claim text.