Fluidic force discrimination

A method for separating or sorting of populations of ligands by fluidics force discrimination, comprising

• selecting a substrate onto which can be bonded one or more types of receptor molecules;
• reacting said substrate with said receptor molecules, wherein said receptors are bound to said substrate;
• reacting said substrate with one or more complementary ligands, wherein said ligands bind with their said complementary receptor molecules to form ligand-receptor pairs, said ligand molecules being labeled with a micro-particle;
• and
• applying the fluidic force discrimination method to said substrate, wherein the bonds of the weakest of said complementary ligand-receptor pairs are broken and the released ligands and beads can be separated by flow or other means from the substrate and ligands attached to the substrate,
• wherein said substrate is porous and the fluidic force discrimination comprises flowing a fluid normally through said substrate.

A method for identifying molecular bonds in assay measurement systems, comprising:

• selecting a substrate onto which can be bonded one or more types of receptor molecules;
• reacting said substrate with one or more said molecules, wherein said molecules are bound to said substrate;
• said molecule being labeled with a micro-particle that can be detected;
• applying fluidic force discrimination method, wherein said molecular bonds are broken apart,
• wherein said substrate is porous and the fluidic force discrimination comprises flowing a fluid normally through said substrate.

A method for separating or sorting of populations of molecules by fluidics force discrimination, comprising

• selecting a substrate onto which can be bonded one or more types of receptor molecules;
• reacting said substrate with said molecules, wherein said molecules are bound to said substrate;
• said molecules being labeled with a microparticle;
• and
• applying the fluidic force discrimination method to said substrate, wherein the bonds of the weakest of the molecular bonds are broken and the released molecules can be separated by flow or other means from the substrate and molecules attached to the substrate,
• wherein said substrate is porous and the fluidic force discrimination comprises flowing a fluid normally through said substrate.