US 8,137,970 B2 · Methods for inducing the differentiation of hematopoietic stem cells into megakaryocytes and platelets, and gene controlling the differentiation

Abstract

The present invention relates to a method for inducing the differentiation of CD34+ hematopoietic stem cells into megakaryocytes and platelets, more particularly, a method for inducing the differentiation of CD34+ hematopoietic stem cells into megakaryocytes and platelets comprising the steps of coculturing CD34+ hematopoietic stem cells with stromal cells and adding the compound of Formula 1. Further, the present invention relates to a composition for detecting the differentiation of hematopoietic stem cells into megakaryocytes and platelets, comprising an agent measuring expression level of a gene that is selected from the group consisting of KLF2 (Kiruppel-like factor2), LOC138255 (OTTHUMP00000021439), GDF15 (growth differentiation factor 15) and INHBE (inhibin, betaE), a kit comprising the composition, a method for detecting the differentiation into megakaryocytes and platelets by using the marker genes, a method for regulating the differentiation into megakaryocytes and platelets, and a method for screening a candidate compound that regulates the differentiation into megakaryocytes and platelets.

Claims3

Dependent claims

A method for inducing the differentiation of CD34+ hematopoietic stem cells into megakaryocytes and platelets, comprising the steps of (a) coculturing CD34+ hematopoietic stem cells with stromal' cells;

and (b) adding the compound of the following Formula 1, wherein expression level of a gene selected from the group consisting of KLF2 (Kruppel-like factor2, NM 016270), LOC138255 (OTTHUMP00000021439, NM 001010940), GDF15 (growth differentiation factor 15, NM 004864) and INHBE (inhibin, betaE, NM 031479) is increased in CD34+ hematopoietic stem cells that are differentiated, relative to undifferentiated CD34+ hematopoietic stem cells.

Dependencies are inferred from the claim text.

Facts

Application

12/364,341Filed 2 Feb 2009

Priority

KR 10-2008-006296830 Jun 2008

Published

US 2009/0324583 A131 Dec 2009

Granted

20 Mar 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12Q 1/6881
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for tissue or cell typing, e.g. human leukocyte antigen [HLA]…
A61K 31/661
Phosphorus compounds: phosphorus acids or esters thereof not having P—C bonds, e.g. fosfosal, dichlorvos, malathion or mevinphos
A61P 7/04
Drugs for disorders of the blood or the extracellular fluid: antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
C12N 5/0644
Cells from the blood or the immune system: platelets; Megakaryocytes

Methods for inducing the differentiation of hematopoietic stem cells into megakaryocytes and platelets, and gene controlling the differentiation

Life of this patent

Methods for inducing the differentiation of hematopoietic stem cells into megakaryocytes and platelets, and gene controlling the differentiation

Life of this patent

Abstract

Claims3