Method of producing three-dimensional tissue and method of producing extracellular matrix used in the same

Abstract

The present invention provides a novel method of producing a three-dimensional tissue by which cell lamination can be carried out easily. According to the method, a three-dimensional tissue in which cell layers are laminated with an extracellular matrix intervening between each pair of the adjacent cell layers is produced by: (A) forming a cell layer on a substrate; (B) bringing the cell layer formed on the substrate into contact with a solution containing a first substance and a solution containing a second substance alternately, thus forming, on the cell layer, an extracellular matrix in which the first substance and the second substance are laminated alternately; and (C) culturing a cell on the extracellular matrix to form a further cell layer. In the present invention, a combination of the first substance and the second substance is (a) a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence or (b) a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.

Dependencies are inferred from the claim text.