Plant-specific genetic elements and transfer cassettes for plant transformation
AssigneeJ.R. Simplot Company
InventorCaius Rommens
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Abstract
The present invention provides nucleic acid molecules and sequences, particularly those identified and obtained from plants, that are useful for transferring and integrating one polynucleotide into another via plant transformation techniques.
Claims27
A transfer-DNA, comprising a first polynucleotide positioned between, operatively linked to, and delineated by, a second and third polynucleotide, wherein (i) the first polynucleotide comprises at least one promoter operatively linked to a desired polynucleotide, (ii) the second polynucleotide is (a) a transfer-DNA Right Border, (b) a plant-derived transfer-DNA border sequence, or (c) a homoendonuclease recognition site, and (iii) the third polynucleotide is an origin of conjugative plasmid DNA transfer that contains a cleavage site and functions as a transfer-DNA Left Border replacement.
A cassette, comprising a first polynucleotide, which comprises a non-autonomous transposable element, positioned between a second and third polynucleotide, wherein the second and third polynucleotides can mediate either single-stranded or double-stranded DNA cleavage, wherein the cassette is not a plant transformation cassette.
Dependencies are inferred from the claim text.