Lactic acid-producing yeast cells having nonfunctional L- or D-lactate:ferricytochrome C oxidoreductase cells
AssigneeCargill, Incorporated
InventorsMatthew Miller, Pirkko Suominen, Aristos Aristidou, Benjamin Matthew Hause, Pim Van Hoek, Catherine Asleson Dundon
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Abstract
Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced L- or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
Claims7
A genetically modified yeast cell having (a) at least one functional exogenous lactate dehydrogenase (LDH) gene integrated into its genome and (b) a deletion or disruption of at least one native L- or D-lactate:ferricytochrome c oxidoreductase gene.
Dependencies are inferred from the claim text.