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AssigneeCj Cheiljedang Corporation
InventorsSeong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee
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The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.
A method of preparing food grade tagatose comprising culturing an immobilized cell of a recombinant GRAS (Generally recognized as safe) strain that expresses thermophilic arabinose isomerase in an active form after transformation with an expression vector which contains a gene encoding the thermophilic arabinose isomerase originating from a thermophilic Geobacillus sp. and contacting the cultured immobilized cell with a substrate that is converted into tagatose by the thermophilic arabinose isomerase, wherein the expression vector is shuttle vector pHT01 originating from Bacillus sp. or shuttle vector pCJ-1 originating from Corynebacterium sp.
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