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US 8,137,942 B2Granted
Method of preparing a modified surface
AssigneePacific Biosciences of California, Inc.
InventorsDaniel Roitman, Paul Peluso, Mathieu Foquet
Life of this patent
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Abstract
Reactive surfaces, substrates and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide low density reactive groups preferably on an otherwise non-reactive surface for use in different applications including single molecule analyses.
Claims12
A method of preparing a modified surface, comprising:
- providing a surface to be modified;
- contacting the surface to be modified with a surface modifying composition, wherein the surface modifying composition comprises:
- a first surface modifying agent coupled to a reactive moiety, and a second surface modifying agent not coupled to the reactive moiety;
- and
- the first surface modifying agent and second surface modifying agent are present in the surface modifying composition at a ratio that produces the modified surface, wherein the reactive moieties are present on the modified surface at a density of between about 1 reactive moiety per 50,000 nm2 and 1 reactive moiety per 100 nm2;
- and
- coupling an enzyme to the reactive moiety on the surface.
4 more elements
Dependencies are inferred from the claim text.