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US 8,137,935 B2Granted
Method for isolating endotoxins
AssigneeUniversite Paris-Sud Xi
InventorMartine Caroff
Life of this patent
Estimating the term…
Abstract
The invention concerns a novel method for extracting endotoxins from bacteria, and the use of said method for preparing compositions comprising endotoxins or derivatives thereof designed for human or animal use (scientific, medical usage).
Claims57
A method for isolating one or more endotoxins, comprising:
- suspending a group of bacterial cells or a culture supernatant, wherein the bacterial cells or the culture supernantant contain the endotoxins, in a mixture of solvents containing:
- 10 to 90% of a solvent chosen from:
- linear or branched aliphatic acids containing from 3 to 6 carbon atoms, and
- 90 to 10% of a basic aqueous solution of an aliphatic amine containing from 0 to 12 carbon atoms;
- stirring the suspended bacterial cells or a culture supernatant to produce a solution of bacterial endotoxins in the mixture of solvents containing suspended particles of cell debris;
- and
- separating the particles from the solution by (1) filtration, (2) centrifugation and recover of the supernatant or (3) centrifugation followed by filtration of the supernatant, to produce the isolated endotoxins,
- wherein the endotoxins are not obtained in combination with silica.
5 more elements
A method for isolating one or more endotoxins, comprising:
- suspending 1 gram or more of a group of bacterial cells or a culture supernatant containing 1 gram or more of bacterial cells, wherein the bacterial cells or the culture supernantant contain the endotoxins, in a mixture of solvents containing:
- 10 to 90% of a solvent chosen from:
- linear or branched aliphatic acids containing from 3 to 6 carbon atoms, and
- 90 to 10% of a basic aqueous solution of an aliphatic amine containing from 0 to 12 carbon atoms;
- stirring the suspended bacterial cells or a culture supernatant to produce a solution of bacterial endotoxins in the mixture of solvents containing suspended particles of cell debris;
- separating the particles from the solution by (1) filtration, (2) centrifugation followed by recovery of the resulting supernatant or (3) centrifugation followed by filtration of the resulting supernatant;
- and
- eliminating solvents from the filtrate or supernatant containing endotoxins.
5 more elements
A method for isolating one or more endotoxins, comprising:
- suspending a group of bacterial cells or a culture supernatant, wherein the bacterial cells or the culture supernantant contain the endotoxins, in a mixture of solvents containing:
- 10 to 90% of a solvent chosen from:
- linear or branched aliphatic acids containing from 3 to 6 carbon atoms, and
- 90 to 10% of a basic aqueous solution of an aliphatic amine containing from 0 to 12 carbon atoms;
- stirring the suspended bacterial cells or a culture supernatant to produce a solution of baterial endotoxins in the mixture of solvents containing suspended particles of cell debris;
- separating the particles from the solution by (1) filtration, (2) centrifugation followed by recovery of the resulting supernatant or (3) centrifugation followed by filtration of the resulting supernatant;
- and
- eliminating solvents from the filtrate or supernatant containing endotoxins,
- wherein the method is conducted on an industrial scale.
6 more elements
A method for isolating one or more endotoxins, comprising:
- suspending a group of bacterial cells or a culture supernatant, wherein the bacterial cells or the culture supernantant contain the endotoxins, in a mixture of solvents containing:
- 10 to 90% of a solvent chosen from:
- linear or branched aliphatic acids containing from 3 to 6 carbon atoms, and
- 90 to 10% of a basic aqueous solution of an aliphatic amine containing from 0 to 12 carbon atoms;
- stirring the suspended bacterial cells or a culture supernatant to produce a solution pf bacterial endotoxins in the mixture of solvents containing suspended particles of cell debris;
- separating the particles from the solution by centrifugation and recovery of the resulting supernatant;
- and
- eliminating solvents from the supernatant containing endotoxins.
5 more elements
Dependencies are inferred from the claim text.