Regeneration and neogenesis of retinal photoreceptor cell using Otx2 gene
AssigneeJapan Science and Technology Agency
InventorTakahisa Furukawa
Life of this patent
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Abstract
The present invention provides a medicine, comprising (a) an Otx2 protein or its partial peptide, or a salt thereof, or (b) a DNA or an RNA encoding an Otx2 protein or its partial peptide. The present medicine is useful as an agent for preventing, treating or suppressing progression of a retinal disease including retinal degeneration. In addition, the present medicine is useful, for example, as an agent for inducing differentiation from a retinal stem cell into a retinal photoreceptor cell, in the transplantation of a cell into the retina of patients suffering from retinal diseases.
Claims13
A method for inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, which method comprises:
- introducing a DNA or an RNA encoding an Otx2 protein into an eye ball tissue-derived cell, an embryonic stem cell, a neural stem cell or a neural precursor cell;
- and
- culturing the resulting cell containing the DNA or RNA encoding the Otx2 protein to express an Otx2 protein, or increase an amount of expression of an Otx2 protein,
- wherein the Otx2 protein is
- (1) a protein having the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5;
- or
- (2) a protein having
- (i) an amino acid sequence in which 1 or 2 amino acid(s) in the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5 is/are deleted,
- (ii) an amino acid sequence in which 1 or 2 amino acid(s) is/are added to the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5,
- (iii) an amino acid sequence in which 1 or 2 amino acid(s) in the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5 is/are substituted with other amino acid(s), or
- (iv) an amino acid sequence as a combination thereof,
- the protein having amino acid sequence (i), (ii), (iii) or (iv) having an action of inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, wherein said action is equivalent to that of the protein having the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
A method for inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, which method comprises:
- introducing a DNA or an RNA encoding an Otx2 protein into an eye ball tissue-derived cell, an embryonic stem cell, a neural stem cell or a neural precursor cell;
- and
- culturing the resulting cell containing the DNA or RNA encoding an Otx2 protein to express an Otx2 protein, or increase an amount of expression of an Otx2 protein,
- wherein the DNA encoding an Otx2 protein has
- (1) the nucleotide sequence represented by SEQ ID NO:
- 2, SEQ ID NO:
- 4 or SEQ ID NO:
- 6;
- or
- (2) a nucleotide sequence having at least 95% homology with the nucleotide sequence represented by SEQ ID NO:
- 2, SEQ ID NO:
- 4 or SEQ ID NO:
- 6 and encoding an Otx2 protein having an action of inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, wherein said action is equivalent to that of the protein having the amino acid sequence represented by SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.