Mammalian expression vector pUHAB
AssigneeSchering Corporation
InventorDeba P. Saha
Life of this patent
Estimating the term…
Abstract
The present invention relates to the construction and utilization of a new mammalian expression vector that contains a unique multiple cloning site (MCS), designated pUHAB. The pUHAB vector comprises a high copy replication origin (ColE1), a drug resistance gene (TK-Hygromycin), and a human cytomegalovirus promoter operably associated with a unique intron (hCMV/intron). Further, pUHAB comprises a selectable marker conferring resistance to kanamycin in bacterial cells, and a phage f1(+) region. pUHAB can be used to transiently or stably express cloned genes when transfected into mammalian cells. The invention also encompasses kits and host cells and cell lines comprising pUHAB, and methods of producing a recombinant protein using pUHAB.
Claims4
A method for producing a recombinant polypeptide in an isolated host cell, comprising introducing a polynucleotide encoding the polypeptide into a multiple cloning site of a vector, wherein the vector comprises the nucleotide sequence set forth in SEQ ID NO:
- 1, and introducing said vector comprising the polynucleotide encoding the polypeptide into a host cell under conditions which allow for expression of the polypeptide in said cell.
Dependencies are inferred from the claim text.