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US 8,137,929 B2Granted
Basic protein purification tags from thermophilic bacteria
AssigneeNovo Nordisk Health Care AG
InventorsAllan Christian Shaw, Susanne Bang, Jing Su
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Abstract
The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues.
Claims12
A method for purification of a recombinant target protein expressed in a eukaryotic or prokaryotic host cell comprising:
- (a) isolating an expressed tagged protein from the host cell, wherein the expressed tagged protein comprises a target protein, the purification tag of SEQ ID NO:
- 24, and a linker sequence comprising a cleavage site for in vitro cleavage of the target protein from the purification tag;
- (b) subjecting the expressed tagged protein to cation-exchange chromatography purification;
- (c) cleaving the target protein from the purification tag;
- and
- (d) isolating the target protein.
3 more elements
Dependencies are inferred from the claim text.