Multi-wavelength analyses of sol-particle specific binding assays
AssigneeAbaxis, Inc.
InventorsVincent Chiang, Rajesh K. Mehra
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Abstract
The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to identify intermolecular interactions between the analyte and its binding partner.
Claims21
A method for determining the presence or absence of a complex of a first reagent and a second reagent in a mixture comprising
- determining a first absorbance value at a first wavelength and a second absorbance value at a second wavelength upon mixing the first reagent with the second reagent, wherein the first reagent or the second reagent is conjugated to a metal nanoparticle or nanoshell, and wherein the first wavelength is within the absorbance wavelength region of the absorption spectrum for the metal nanoparticle or nanoshell and the second wavelength is within a scattering wavelength region of the absorption spectrum for the metal nanoparticle or nanoshell;
- determining the ratio of the first absorbance value to the second absorbance value;
- and
- comparing the ratio of the absorbance values to a pre-determined value, wherein the comparison is indicative of the presence of the complex of the first reagent and the second reagent.
Dependencies are inferred from the claim text.