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US 8,137,904 B2Granted
Light emitting microorganisms and cells for diagnosis and therapy of diseases associated with wounded or inflamed tissue
AssigneeGenelux Corporation
InventorsAladar A. Szalay, Shahrokh Shabahang, Yong A. Yu
Life of this patent
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Abstract
Described is the use of a microorganism or cell containing a DNA sequence encoding a detectable protein or a protein capable of inducing a detectable signal, e.g., a luminescent or fluorescent protein for the preparation of a diagnostic composition for diagnosis and/or visualization of wounded or inflamed tissue or a disease associated therewith. Moreover, therapeutic uses are described, wherein the microorganism or cell additionally contain an expressible DNA sequence encoding a protein suitable for therapy, e.g. an enzyme causing cell death or digestion of debris.
Claims24
A method for diagnosis by imaging wounded or inflamed tissue inside of a live subject, comprising:
- identifying a subject suspected of having an internal wound or inflammation to be tested for the presence or absence of internal wounded tissue or internal inflamed tissue;
- systemically administering to the subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium encodes a protein that is detectable by imaging in the subject or encodes a protein that induces a signal detectable by imaging;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, imaging the detectable bacterium inside of the live subject to detect accumulation of the bacterium in the subject, and thereby imaging wounded or inflamed tissues inside of the live subject, wherein imaging the accumulation indicates the location of wounded tissue or inflamed tissue inside of the subject.
5 more elements
A method for detecting wounded or inflamed tissue inside a subject, comprising:
- identifying a subject to be tested for the presence or absence of wounded tissue or inflamed tissue therein;
- systemically administering to the subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium is detectable by imaging in the subject;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, imaging the detectable bacterium inside of the subject to detect accumulation of the bacterium in the subject, and thereby detecting wounded or inflamed tissues inside of the subject, wherein:
- detection of the accumulation indicates the location of wounded tissue or inflamed tissue inside of the subject;
- and the bacterium encodes a protein that induces a signal detectable by magnetic resonance imaging (MRI) or that binds to contrasting agent, chromophore or a ligand.
7 more elements
A method for detecting wounded or inflamed tissue inside of a subject, comprising:
- identifying a subject to be tested for the presence or absence of wounded tissue or inflamed tissue therein;
- systemically administering to the subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium is detectable by imaging in the subject;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, imaging the detectable bacterium inside of the subject to detect accumulation of the bacterium in the subject, and thereby detecting wounded or inflamed tissues inside of the subject, wherein:
- detection of the accumulation indicates the location of wounded tissue or inflamed tissue inside of the subject;
- the bacterium encodes a protein(s) for the therapy of the detected imaged wounded or inflamed tissue;
- and
- the subject for whom the presence or absence of wounded tissue or inflamed tissue is detected has an atherosclerotic disease.
9 more elements
A method for detecting wounded or inflamed tissue inside of a subject, comprising:
- identifying a subject to be tested for the presence or absence of wounded tissue or inflamed tissue therein;
- systemically administering to the subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium is detectable by imaging in the subject;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, imaging the detectable bacterium inside of the subject to detect accumulation of the bacterium in the subject, and thereby detecting wounded or inflamed tissues inside of the subject, wherein:
- detection of the accumulation indicates the location of wounded tissue or inflamed tissue inside of the subject;
- and
- the detecting is performed by magnetic resonance imaging (MRI).
8 more elements
A method for detecting wounded or inflamed tissue inside of a subject, comprising:
- identifying a subject to be tested for the presence or absence of wounded tissue or inflamed tissue therein;
- systemically administering to the subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium is detectable by imaging in the subject;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, imaging the detectable bacterium inside of the subject to detect accumulation of the bacterium in the subject, and thereby detecting wounded or inflamed tissues inside of the subject, wherein:
- detection of the accumulation indicates the location of wounded tissue or inflamed tissue inside of the subject;
- the disease is an atherosclerotic disease;
- and
- the therapeutic protein is selected from among a lipase, protease, lysozyme, proapoptotic factor and PPAR-agonist.
9 more elements
A method for detecting wounded or inflamed tissue inside of a subject, comprising:
- intravenously or intramuscularly administering to a subject in whom the presence or absence of a wounded tissue or inflamed tissue is to be detected, a bacterium, wherein:
- the bacterium is detectable in the subject;
- the bacterium replicates in the subject;
- the bacterium is not pathogenic to the subject and is recognized by the immune system of the subject;
- the bacterium is not targeted;
- and
- after a sufficient time for the bacterium to accumulate in wounded or inflamed tissues inside of the subject, monitoring or imaging the detectable bacterium inside of the subject to detect accumulation of the bacterium in the subject, and thereby detecting wounded or inflamed tissues inside of the subject.
4 more elements
Dependencies are inferred from the claim text.