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US 8,137,707 B2Granted
Method of obtaining an active ingredient increasing cutaneous cell and tissue longevity, active ingredients and compositions
AssigneeSociete Industrielle Limousine D'Application Biologique Dite Silab
InventorJean Paufique
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Abstract
Method for obtaining of an active principle from Myrtus communis in order to increase the longevity of the cells and tissues of the skin.
Claims8
A method of increasing cutaneous tissue longevity and cutaneous cellular longevity, comprising applying a Myrtus communis active principle having:
- a percentage of dry materials ranging between 20 and 200 g/L,
- a pH ranging between 3.0 and 6.0,
- a content of total sugars ranging between 5 and 64 g/L,
- and a content of total phenols ranging between 0.5 and 10.5 g/L
- to human skin, wherein the active principle is obtained by a method comprising:
- (a) solubilizing Myrtus communis leaf powder in water in acid medium to form a solution,
- (b) carrying out simultaneous and/or successive enzymatic hydrolysis/hydrolyses on the solution of (a),
- (c) separating the soluble and insoluble phases of the hydrolyzed solution of (b) by filtration, centrifugation or decantation, to obtain an enzyme-containing soluble phase,
- (d) inactivating the soluble phase of (c) by thermal treatment,
- (e) purifying by clarification, thereby providing an active fraction,
- (f) concentrating the active fraction to provide a concentrated solution of the active fraction, and
- (g) filtering the concentrated solution.
9 more elements
Dependencies are inferred from the claim text.