Biomimetic composition reinforced by a polyelectrolytic complex of hyaluronic acid and chitosan
AssigneeTissue Engineering Consultants, Inc.
InventorJohn H. Brekke
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Abstract
The present invention relates to a three dimensional, malleable cell culture composition and method of forming the same comprising hyaluronic acid, chitosan and a polyelectrolytic complex of hyaluronic acid and chitosan. These three components in combination constitute an initial microenvironment for support of stromal cells, and their undifferentiated mesenchymal cell progeny. The tissue engineering device and method of forming the same comprising hyaluronic acid and chitosan and the use of said device with compositions of pluripotent cells and various formulations of cell culture media for repair of tissues is disclosed.
Claims21
A fluid mass composition comprising:
- unreacted protonated chitosan hydrogel;
- unreacted hyaluronic acid viscoelastic gel;
- and
- polyelectrolytic complex fibers, wherein said polyelectrolytic complex fibers form a three dimensional network throughout the fluid mass composition that surrounds and penetrates the unreacted protonated chitosan hydrogel and the unreacted hyaluronic acid viscoelastic gel and maintains homogeneous regions of the unreacted protonated chitosan hydrogel and the unreacted hyaluronic acid viscoelastic gel.
Dependencies are inferred from the claim text.