Transgenic reptiles
AssigneeNorth Carolina State University
InventorsPaul E. Mozdziak, James N. Petitte
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Abstract
A transgenic animal such as a transgenic snake or other reptile that expresses a heterologous expression product is described, along with methods of making the same. In general, the animal comprises cells containing a sequence encoding the heterologous expression product. The sequence encoding the heterologous expression product is integrated into the genome of the animal (e.g., in some or all cells thereof, and in some embodiments into germ cells thereof). The sequence encoding the heterologous expression product is, in general, operatively associated with an expression sequence or promoter. The animals are useful for, among other things, testing of repellents, testing of toxicological compounds, as teaching aids, for venom production, etc.
Claims12
A transgenic reptile that expresses a heterologous protein expression product, comprising cells containing an expression sequence operably linked to a sequence encoding said heterologous protein expression product, wherein the sequence encoding the expression product is integrated into the genome of the reptile, and wherein the heterologous protein expression product is expressed in detectable quantities in said reptile.
A transgenic reptile that expresses a heterologous expression product, comprising cells containing an expression sequence operably linked to a sequence encoding said heterologous expression product, wherein the sequence encoding the expression product is integrated into the genome of the reptile;
- wherein said expression product is a fluorescent or luminescent protein and said reptile has a phenotype of fluorescence or luminescence not found in the corresponding wild-type reptile;
- and wherein said reptile is selected from the group consisting of snakes, lizards, turtles, tortoises, and crocodilians.
Dependencies are inferred from the claim text.