Tissue specific promoters
AssigneeKws Saat AG
InventorsReinhard Hehl, Dorothee Kloos, Dietmar Juergen Stahl
Life of this patent
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Abstract
The invention relates to tissue specific promoters which can be used in plants for one or more of the following purposes: a. altering carbohydrate metabolism b. preventing memory substance loss c. expression of an invertase inhibitor d. expression of a fructosyl transferase e. expression of a levan sucrase f. expression of genes coding for transported proteins for N-compounds g. expression of characteristics which increase resistance/tolerance with respect to pathogens.
Claims22
An isolated promoter, which is active in above-ground plant parts, comprising a nucleic acid sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3;
- b. the polynucleotide sequence complementary to the full length of SEQ ID NO:
- 3;
- c. a fragment of SEQ ID NO:
- 3 providing promoter activity in above-ground plant parts;
- and
- d. a fragment of a sequence complementary to the full length of SEQ ID NO:
- 3, wherein said fragment provides promoter activity in above-ground plant parts.
A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter, which is active in above-ground plant parts, that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3 having a promoter activity;
- b. the polynucleotide sequence complementary to the full length of SEQ ID NO:
- 3;
- c. a fragment of SEQ ID NO:
- 3 providing promoter activity in above-ground plant parts;
- and
- d. a fragment of a sequence complementary to the full length of SEQ ID NO:
- 3, wherein said fragment provides promoter activity in above-ground plant parts.
A transgenic plant comprising a promoter that is active in above-ground plant parts and that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3 having a promoter activity;
- b. a sequence complementary to the full length of SEQ ID NO:
- 3;
- c. a fragment of SEQ ID NO:
- 3 providing promoter activity in above-ground plant parts;
- and
- d. a fragment of a sequence complementary to the full length of SEQ ID NO:
- 3, wherein said fragment provides promoter activity in above-ground plant parts.
A method of:
- a. amending carbohydrate metabolism,
- b. avoiding loss of storage substance,
- c. increasing at least one of resistance and tolerance towards a pathogen, or
- d. expressing from a transgene:
- an invertase inhibitor,
- a fructosyl transferase,
- a levan sucrose, or
- a transporter protein for a N-compound, in a transgenic plant, said method comprising:
- (1) transforming a plant cell with a promoter, which is active in above-ground plant parts, wherein said promoter is operably linked to said transgene, and
- (2) subsequently regenerating a transgenic plant from said plant cell,
- wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- i. SEQ ID NO:
- 3;
- ii. the polynucleotide sequence complementary to the full length of SEQ ID NO:
- 3;
- iii. a fragment of SEQ ID NO:
- 3 providing promoter activity in above-ground plant parts;
- and
- iv. a fragment of a sequence complementary to the full length of SEQ ID NO:
- 3, wherein said fragment provides promoter activity in above-ground plant parts
An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3;
- and
- b. the polynucleotide sequence complementary to the full length of SEQ ID NO:
- 3.
A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3 having a promoter activity;
- and
- b. the polynucleotide sequence complementary to the full length of SEQ ID NO:
- 3.
A transgenic plant comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a. SEQ ID NO:
- 3 having a promoter activity;
- and
- b. the polynucleotide sequence complementary to the full length of SEQ ID NO:3.
A method of:
- a. amending carbohydrate metabolism;
- b. avoiding loss of storage substance;
- c. increasing at least one of resistance and tolerance towards a pathogen;
- or
- d. expressing from a transgene:
- an invertase inhibitor,
- a fructosyl transferase,
- a levan sucrose, or
- a transporter protein from an N-compound, in a transgenic plant, said method comprising:
- (1) transforming a plant cell with a promoter, wherein said promoter is operably linked to said transgene;
- and
- (2) subsequently regenerating a transgenic plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- i. SEQ ID NO:
- 3;
- and
- ii. the polynucleotide sequence complementary to SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.