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AssigneeThe Johns Hopkins University School of Medicine
InventorsJean-Pierre Issa, Stephen Baylin, Minoru Toyota
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The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated PcG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers. A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluating the responses of a cell to an agent. A kit is useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones.
A kit comprising a carrier means containing one or more containers comprising a membrane, wherein the membrane has a nucleic acid sequence, and the nucleic acid sequence is selected from the group consisting of SEQ ID NO:
An isolated nucleic acid selected from the group consisting of SEQ ID NO:
An isolated nucleic acid sequence comprising at least one methylated CpG dinucleotide, wherein the isolated nucleic acid sequence is selected from the group consisting of SEQ ID NOs:
Dependencies are inferred from the claim text.