Peptide nucleic acids conjugated with multi-amine linkers and nucleic acid detecting device using the same

A method to prepare a PNA conjugated with multi-amine linkers, the method comprising:

• sequentially reacting a monomer (Chemical Formula 3) having multi-amine protected functionality, with a PNA oligomer derivative (Chemical Formula 2) to obtain the PNA (Chemical Formula 1) wherein the multi-amine linkers have been conjugated,
• wherein in the Formulas, Z represents a PNA oligomer having 8-30 nucleobases;
• wherein A is bonded to N-terminal of the PNA oligomer and is optional, and when present represents
• wherein D represents alkylene having 8-200 carbon atoms, and one or more carbon atoms of alkylene may be substituted by nitrogen, oxygen or carbonyl;
• LI, L2 and L3 independently represent a chemical bond, or an alkylene having 1˜10 carbon atom(s), wherein the carbon atoms in the alkylene may be further substituted by one or more oxygen atom(s);
• X represents CH or N;
• Y represents an amine protective group;
• m represents an integer from 2 to 10;
• and
• n is 0 or 1.