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US 8,133,731 B2Granted
Production of primate neural stem cells through expression of pax6
AssigneeWisconsin Alumni Research Foundation
InventorsSu-Chun Zhang, Xiaoqing Zhang
Life of this patent
Estimating the term…
Abstract
A transcription factor both necessary and sufficient for human neuroectoderm specification, Pax6, as well as applications thereof, is disclosed.
Claims11
A method of creating a population of primate pNSCs from primate embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) comprising the step of:
- introducing a nucleic acid sequence encoding Pax6 into primate ESCs or iPSCs, wherein Pax6 production within the cells is sufficient produce Pax6+Sox1-primate pNSCs.
A method of creating a population of primate regional neural stem cells comprising the steps of:
- (a) introducing a nucleic acid sequence encoding Pax6 into primate ESCs or iPSCs, wherein Pax6 production within the cells is sufficient produce Pax6+Sox1− primate pNSCs;
- (b) suppressing Pax6 production;
- and
- (c) differentiating the cells of step (b) into regional neural stem cells.
1 more element
A method of creating a population of primate pNSCs from primate regional neural stem cells comprising the step of:
- introducing a nucleic acid sequence encoding Pax6 into isolated primate regional neural stem or progenitor cells, wherein Pax6 production within the cells is sufficient produce Pax6+Sox1− primate pNSCs is sufficient to reprogram the cells to the primate pNSC stage.
Dependencies are inferred from the claim text.