Generation of neural stem cells from undifferentiated human embryonic stem cells
AssigneeES Cell International PTE Ltd.
InventorBenjamin Eithan Reubinoff
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Abstract
The present invention relates to the generation of neural cells from undifferentiated human embryonic stem cells. In particular it relates to directing the differentiation of human ES cells into neural progenitors and neural cells and the production of functioning neural cells and/or neural cells of a specific type. The invention also includes the use of these cells for the treatment of neurological conditions such as Parkinson's disease.
Claims9
A method of generating neural progenitor cells in vitro, comprising
- (a) culturing undifferentiated human embryonic stem (ES) cells in a serum free medium comprising FGF-2 and noggin to generate neural progenitor cells;
- and
- (b) further differentiating said neural progenitor cells in a serum free medium comprising FGF2 for three weeks, said serum free medium being devoid of noggin, thereby generating neural progenitor cells.
A method of generating neural progenitor cells in vitro, the method culturing undifferentiated human embryonic stem (ES) cells in a serum free medium comprising FGF-2 and noggin, said medium being devoid of LIF, thereby generating neural progenitor cells.
Dependencies are inferred from the claim text.