Biomimetic urothelium
AssigneeThe University of York
InventorsWilliam Richard Cross, Jennifer Southgate
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Abstract
Urothelium is the specialized endothelium that lines much of the mammalian urinary tract. This invention relates to the field of in vitro urothelial culture. Previous methods produce tissue lacking much of the functionality of native urothelium. In this invention, stratified, differentiated mammalian urothelium is produced by passaging urothelial cells through a nutrient medium containing serum, and redispersed, before going on in a like medium to form said urothelium.
Claims12
A method of production of stratified, terminally-differentiated human urothelium, the method comprising:
- propagation by serial culture of human urothelial cells in a serum-free nutrient medium;
- replacing the serum-free nutrient medium with a first differentiation cell culture medium that includes whole serum;
- maintaining the urothelial cells in the first differentiation culture medium to form a cell culture having at least partially confluent, non terminally-differentiated urothelial cells;
- disaggregating and dispersing said non terminally-differentiated urothelial cells into a fresh second differentiation cell culture medium that includes whole serum;
- and
- culturing the urothelial cells in the second differentiation culture medium so as to form stratified, terminally-differentiated human urothelium.
A method of production of stratified, terminally-differentiated human urothelium, the method comprising:
- propagation by serial culture of human urothelial cells in a serum-free nutrient medium;
- replacement of the serum-free nutrient medium with a first differentiation cell culture medium that includes at least 5% whole serum;
- maintaining the urothelial cells in the first differentiation cell culture medium to form a cell culture having at least partially confluent, non terminally-differentiated urothelial cells;
- disaggregating and dispersing said non terminally-differentiated urothelial cells into a fresh second differentiation cell culture medium that includes at least 5% whole serum;
- and
- culturing the urothelial cells and increasing the calcium concentration of the second differentiation culture medium so as to form stratified, terminally-differentiated human urothelium.
Dependencies are inferred from the claim text.