Static diffusion cell for diffusion sampling systems

A method of performing an assay, the method comprising:

• depositing a test material on a donor compartment;
• permeating the test material through a diffusion membrane;
• receiving the test material into a receptor compartment that is a single-chambered compartment that includes a first outlet and a second outlet, wherein the diffusion membrane and the donor compartment are positioned over the first outlet, and the second outlet forms a bubble trap and a sampling arm, wherein a bottom surface of the diffusion membrane forms at least a portion of the top surface of the receptor compartment and the first outlet of the receptor chamber is formed such that the portion of the top surface of the receptor compartment formed by the bottom surface of the diffusion membrane inclines upward toward the second outlet, and wherein the first outlet and the second outlet are formed at the top surface of the receptor compartment;
• removing bubbles through a bubble channel that is located in the top surface of the receptor and extends between the first and second outlet;
• obtaining a sample through the sampling arm;
• and
• assaying the sample.

A diffusion sampling system comprising:

• one or more diffusion cells that comprise:
• a donor compartment, a diffusion membrane, a receptor compartment, which is a single-chambered compartment that includes a first outlet and a second outlet that are located in the top surface of the receptor compartment, wherein the diffusion membrane and the donor compartment are positioned over the first outlet;
• and a channel located in the top surface extending between the first and the second outlets;
• and
• a mounting apparatus positioned with the one or more diffusion cells.