Analytical strip and detecting method using the same
AssigneeActherm Inc.
InventorsYi-Jen Wu, Chih-Wei Hsieh, Wen-Pin Hsieh
Life of this patent
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Abstract
An analytical strip and a detecting method using the analytical strip are provided. The analytical strip includes a substrate having a channel thereon. The channel has a first region, a second region and a third region, which are arranged successively. A first antibody is localized in the first region. A saccharide and a peroxidase are localized in the first or second region. A second antibody for recognizing a different epitope of an identical antigen with the first antibody is immobilized in the second region. A substrate reagent including a saccharide oxidase is localized in the third region.
Claims20
An analytical strip, comprising a substrate having at least one channel thereon, wherein the channel includes a first region, a second region and a third region arranged successively, and the first region being used for a fluid sample to be introduced thereinto, the analytical strip being characterized by:
- a first antibody localized in the first region for recognizing an analyte in the fluid sample;
- a saccharide localized in the first or the second region;
- a peroxidase localized in the first or the second region;
- a second antibody immobilized in the second region for recognizing the analyte in the fluid sample, wherein the second antibody and the first antibody are configured to recognize different epitopes of the analyte in the fluid sample;
- and
- a substrate reagent localized in the third region and comprising a saccharide oxidase;
- wherein upon introduction of the fluid sample containing the analyte into the channel, the first antibody, the saccharide and the peroxidase flow along with the fluid sample, and then a part of the peroxidase binds with the first antibody, the analyte and the second antibody so as to be retained in the second region, allowing the other part of the peroxidase to flow along with the fluid sample to the third region, the saccharide arrives at the third region and is catalyzed by the saccharide oxidase so as to produce hydrogen peroxide, and then the hydrogen peroxide reacts with the peroxidase arriving at the third region so as to generate an electrical signal.
A detecting method, comprising steps of:
- providing a fluid sample that contains an analyte;
- providing a substrate having at least one channel including a first region, a second region and a third region arranged successively, wherein the first region allows a fluid sample to be introduced thereinto, and the substrate further comprises:
- a first antibody localized in the first region for recognizing an analyte in the fluid sample,
- a saccharide localized in the first or the second region;
- a peroxidase localized in the first or the second region;
- a second antibody immobilized in the second region for recognizing the analyte in the fluid sample, wherein the second antibody and the first antibody are configured to recognize different epitopes of the analyte in the fluid sample;
- and
- a substrate reagent localized in the third region and comprising a saccharide oxidase;
- introducing the fluid sample into the first region of the channel to make the first antibody, saccharide, and peroxidase flow together with the fluid sample;
- the analyte, the first antibody, the second antibody and a part of the peroxidase binding with each other and retaining in the second region;
- the saccharide, the unbound first antibody, and the other part of the peroxidase unbound to flow to the third region along with the fluid sample so that the saccharide is catalyzed by the saccharide oxidase to produce hydrogen peroxide;
- allowing the peroxidase arriving at the third region to react with the hydrogen peroxide so as to generate an electrical signal;
- and
- detecting the generated electrical signal.
Dependencies are inferred from the claim text.