Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
AssigneeThe Regents of the University of California
InventorAnastasios Melis
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Abstract
The present invention provides methods and compositions for producing isoprene hydrocarbons from microalgae, cyanobacteria, and photosynthetic and non-photosynthetic bacteria.
Claims10
A method of producing isoprene hydrocarbons in a cyanobacteria, the method comprising:
- introducing an expression cassette that comprises a nucleic acid encoding an isoprene synthase that comprises amino acid residues 53-595 of SEQ ID NO:2 into the cyanobacteria, wherein the nucleic acid is codon-optimized for expression in the cyanobacteria and comprises at least 95% identity to the isoprene synthase coding region of SEQ ID NO:5;
- and
- culturing the cyanobacteria under conditions in which the nucleic acid encoding isoprene synthase is expressed and produces isoprene.
A method of producing isoprene hydrocarbons in a microorganism that comprises a heterologous nucleic acid that encodes isoprene synthase, the method comprising:
- mass-culturing cyanobacteria that comprise a heterologous nucleic acid encoding an isoprene synthase that comprises amino acid residues 53-595 of SEQ ID NO:2 operably linked to a promoter, wherein the nucleic acid is codon-optimized for expression in the cyanobacteria and comprises at least 95% identity to the isoprene synthase coding region of SEQ ID NO:5;
- and wherein the cyanobacteria are in an enclosed bioreactor and cultured under conditions in which the isoprene synthase gene is expressed;
- and
- harvesting volatile isoprene hydrocarbons produced by the cyanobacteria.
An isolated nucleic acid encoding an isoprene synthase that comprises amino acid residues 53-595 of SEQ ID NO:2, wherein the nucleic acid is codon-optimized for expression in cyanobacteria and comprises at least 95% identity to the isoprene synthase coding region of SEQ ID NO:5.
Dependencies are inferred from the claim text.