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US 8,133,699 B2Granted
Minimal DNA sequence acting as a chromatin insulator and its use in protein expression
AssigneeMerk Serono S.A.
InventorsPhilippe Chatellard, Markus Imhof
Life of this patent
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Abstract
The invention relates to expression vectors comprising a DNA sequence of 146 bp capable of acting as chromatin insulator, to host cells containing such vectors, to a method of producing a desired polypeptide by using vectors containing said sequence and to the use of said DNA sequence.
Claims19
A method of expressing a gene of interest in a Chinese Hamster Ovary (CHO) cell comprising
- culturing a CHO cell comprising a vector under conditions that allow for the expression of said gene of interest,
- said vector comprising a promoter, a DNA encoding at least one gene of interest and one or more chromatin insulators consisting of SEQ ID NO:
- 1 and wherein said CHO cell expresses the gene of interest.
Dependencies are inferred from the claim text.