Measurement of binding rate of a binding substance and an analyte
AssigneeOrion Diagnostica Oy
InventorKauko Kahma
Life of this patent
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Abstract
A method of measuring the rate of binding of a binding substance and an analyte, for example in an assay such as an immunoassay, uses an initial step of performing ultrasonication sufficient to disrupt binding between the binding substance and the analyte. After cessation of the ultrasonication, measurements are taken to determine the rate of binding at cessation of said ultrasonication or at a predetermined time thereafter. The ultrasonication results in knowledge of the precise time of the start of the binding reaction which provides a better rate measurement.
Claims26
A method of measuring the rate of binding of a binding substance and an analyte, the method comprising:
- disposing the binding substance and the analyte in a medium;
- performing sonication of the medium sufficient to disrupt binding between the binding substance and the analyte;
- and
- ceasing said sonication of the medium and determining the rate of binding at a predetermined time at or after cessation of said sonication by extrapolation from measurements made after cessation of said sonication.
Dependencies are inferred from the claim text.