Stable quantitation and detection of immune response levels with non-zero background peptides
AssigneeThe Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
InventorsSathibalan Ponniah, George E. Peoples, Catherine E. Storrer, Michael Flora
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Abstract
The invention relates to a kit comprising MHC Class I and Class II HLA-coated beads containing specific antigenic peptides for binding to antigen-specific T cells and the appropriate negative control peptides. Also provided are methods for making the coated beads and methods for use. The application of these beads go to the stimulation of peripheral blood cell populations and in vitro-stimulated culture for the elicitation of functional activities such as cell activation and signaling, cytokine secretion, proliferation and cytotoxicity activity.
Claims20
A bead to which a major histocompatibility complex (“MHC”) molecule is covalently or non-covalently coupled, wherein a peptide is associated with said MHC molecule for presentation to a T cell, and wherein said peptide comprises at least two anchor residues, wherein the at least two anchor residues are located at positions 1, 2, or 3 and 8, 9, or 10, and wherein the peptide has the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.