Method for measuring the number of oral Lactobacillus, and a PCR primers-probe set used for the same
AssigneeGC Corporation
InventorsYuko Matsumoto, Yoko Ishihara
Life of this patent
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Abstract
There is provided a real-time PCR assay capable of measuring the number of lactobacillus present in an oral cavity, in a short time. The object can be solved by a method for measuring the number of oral lactobacillus using a combination of a forward primer comprising a oligonucleotide part of at least 15 sequential bases in the base sequence any one of SEQ ID NO: 1 to 4 and a reverse primer comprising a oligonucleotide part of at least 15 sequential bases in the base sequence any one of SEQ ID NO: 5 to 7; and a probe for measuring the number of oral lactobacillus, comprising a oligonucleotide part of at least 10 sequential bases in the base sequence any one of SEQ ID NO: 8 to 15.
Claims7
A primer set for measuring the number of oral lactobacillus, comprising at least one forward primer selected from the group consisting of oligonucleotides of the base sequence of SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEO ID NO:
- 23, SEQ ID NO:
- 24, and SEQ ID NO:
- 25, and at least one reverse primer selected from the group consisting of oligonucleotides of the base sequence of SEQ ID NO:
- 26, SEQ ID NO:
- 27, and SEQ ID NO:
- 28.
Dependencies are inferred from the claim text.