Chemically modified nucleoside 5′-triphosphates for thermally initiated amplification of nucleic acid
AssigneeTriLink BioTechnologies, LLC
InventorsAlexandre Lebedev, Inna Koukhareva
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Abstract
Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and/or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
Claims63
A method of replicating nucleic acids, said method comprising:
- replicating nucleic acid, wherein at least one NTP added to a replication reaction mixture comprises a thermally labile 3′-substitution group and wherein said replication reaction mixture does not contain chemical reagents for removal of said thermally labile 3′-substitution group.
A method for improving specificity of replicating nucleic acids, said method comprising:
- replicating nucleic acid, wherein at least one NTP added to a replication reaction mixture comprises a thermally labile 3′-substitution group and wherein said replication reaction mixture does not contain chemical reagents for removal of said thermally labile 3′-substitution group.
A method for improving efficiency of replicating nucleic acids, said method comprising:
- replicating nucleic acid, wherein at least one NTP added to a replication reaction mixture comprises a thermally labile 3′-substitution group and wherein said replication reaction mixture does not contain chemical reagents for removal of said thermally labile 3′-substitution group.
Dependencies are inferred from the claim text.