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US 8,133,493 B2Granted
Regulated attenuation of live vaccines to enhance cross-protective immunogenicity
AssigneeWashington University
InventorRoy Curtiss, III
Life of this patent
Estimating the term…
Abstract
A live attenuated derivative of a pathogenic bacterium intended for use as a vaccine.
Claims6
A live attenuated strain of Salmonella comprising
- (a) a regulatable araCPBAD promotor that is operably linked to a fur gene, wherein said fur gene is expressed when said attenuated strain is in the intestinal tract of an individual and said fur gene is not expressed when said attenuated strain is within internal tissues of an individual and wherein non- expression of said fur gene in vivo causes synthesis of iron regulated outer membrane proteins (IROMPs);
- and
- (b) a means for regulating synthesis of an LPS O-antigen, wherein said LPS O-antigen ceases to be synthesized in vivo, exposing an LPS core oligosaccharide antigen that is conserved among Salmonella species;
- wherein said attenuated strain is a Salmonella typhimurium strain and has enhanced ability to induce immune responses against Salmonella typhimurium and Salmonella enteritidis species.
1 more element
A live attenuated strain, wherein said strain is a Salmonella typhimurium comprising
- (a) a ΔPfur::TT araCPBADfur deletion-insertion mutation;
- and
- (b) a Δpmi mutation.
Dependencies are inferred from the claim text.