Recombinant bacteria having the ability to metabolize sucrose
AssigneeE.I. du Pont de Nemours and Company
InventorTina K. Van Dyk
Life of this patent
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Abstract
Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence from Pseudomonas fluorescens Pf5 (ATCC® BAA-477) encoding a polypeptide having sucrose transporter activity and a nucleotide sequence from Pseudomonas fluorescens Pf5 (ATCC® BAA-477) encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
Claims10
A recombinant bacterium comprising in its genome or on at least one recombinant construct:
- (a) a nucleotide sequence encoding a polypeptide having sucrose transporter activity, said polypeptide having at least 95% sequence identity, based on a Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NO:24;
- and
- (b) a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity, said polypeptide having at least 95% sequence identity, based on a Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NO:26;
- wherein (a) and (b) are each operably linked to the same or a different promoter, further wherein said recombinant bacterium is capable of metabolizing sucrose.
Dependencies are inferred from the claim text.