L-glutamic acid-producing bacterium and method for producing L-glutamic acid
AssigneeAjinomoto Co., Inc.
InventorsMika Moriya, Hiroshi Izui, Eiji Ono, Kazuhiko Matsui, Hisao Ito, Yoshihiko Hara
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Abstract
L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Pantoea or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.
Claims7
A method for producing L-glutamic acid, comprising:
- culturing a microorganism in a liquid culture medium for a time and under conditions suitable for the production and accumulation of L-glutamic acid in the culture medium wherein production of L-glutamic acid by said microorganism is increased relative to that in the corresponding wild-type microorganism, and
- collecting the L-glutamic acid from the culture medium;
- wherein said microorganism belongs to the genus Pantoea or Serratia and has
- (1) an ability to produce L-glutamic acid in a culture medium, and
- (2) at least one of the following properties:
- (a) the microorganism has an increased activity of at least one enzyme selected from the group consisting of citrate synthase, phosphoenolpyruvate carboxylase, and glutamate dehydrogenase;
- and
- (b) the microorganism has decreased activity of α-ketoglutarate dehydrogenase.
Dependencies are inferred from the claim text.