Back to results
US 8,128,966 B2Granted
Modified pectins, compositions and methods related thereto
AssigneeLa Jolla Pharmaceutical Company
InventorsMark Staples, James Rolke
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions of modified pectin and for preparing and using them.
Claims29
A process for manufacturing a biologically active filtered pectin, comprising:
- (a) passing an aqueous solution comprising 1-20 mg/mL modified or unmodified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of less than 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC50 less than 200 μg/mL in a cancer cell apoptosis model;
- and (b) collecting said biologically active filtered pectin.
A process for manufacturing a partially de-esterified biologically active pectin, comprising:
- (a) treating a first aqueous solution comprising 1-20 mg/ml modified or unmodified pectin with acid, base, or both to partially de-esterify the pectin,
- (b) neutralizing the first aqueous solution to form a second aqueous solution comprising partially de-esterified pectin,
- (c) passing the second aqueous solution comprising partially de-esterified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of less than 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises filtered partially de-esterified biologically active pectin having an average molecular weight from 10 kDa to 250 kDa and an IC50 less than 200 μg/mL in a cancer cell apoptosis model, and wherein the degree of esterification in the resulting partially de-esterified biologically active pectin is between 10% and 60%, and
- (d) collecting said filtered partially de-esterified biologically active pectin.
1 more element
A process for manufacturing a biologically active filtered pectin, comprising:
- (a) passing an aqueous solution comprising 1-20 mg/mL modified or unmodified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of 0.1 μm to 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC50 less than 200 μg/mL in a cancer cell apoptosis model;
- and (b) collecting said biologically active filtered pectin.
A process for manufacturing a partially de-esterified biologically active filtered pectin, comprising:
- (a) treating a first aqueous solution comprising 1-20 mg/ml modified or unmodified pectin with acid, base, or both to partially de-esterify the pectin,
- (b) neutralizing the first aqueous solution to form a second aqueous solution comprising partially de-esterified pectin,
- (c) passing the second aqueous solution comprising partially de-esterified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of 0.1 μm to 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises filtered partially de-esterified biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC50 less than 200 μg/mL in a cancer cell apoptosis model, and wherein the degree of esterification in the resulting partially de-esterified pectin is between 10% and 60%, and
- (d) collecting said partially de-esterified biologically active filtered pectin.
1 more element
Dependencies are inferred from the claim text.