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US 8,124,411 B2Granted
Methods for producing transgenic plants
AssigneeMonsanto Technology LLC
InventorsAnisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
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Abstract
A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
Claims12
A method for producing a transgenic corn plant comprising the steps of:
- a) obtaining a transformable corn explant;
- b) co-culturing the transformable corn explant;
- c) selecting a transformed corn cell from the transformable corn explant on a selection medium;
- and
- d) regenerating the transformed corn cell into a plant on a regeneration medium, wherein steps b), c), and d) are performed in a single container, wherein the explant is selected from the group consisting of a callus, an embryo, and a cell suspension.
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Dependencies are inferred from the claim text.