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US 8,124,340 B2Granted
Methods for enrichment of selected RNA molecules
AssigneeQuanta Biosciences
InventorAyoub Rashtchian
Life of this patent
Estimating the term…
Abstract
Improved methods of studying RNA molecules are provided. In particular, methods of treating mixtures of RNA molecules so as to enrich the mixture for a desired type of RNA molecule are provided. For example, the methods permit depletion of mRNA from complex mixtures to facilitate study of microRNAs in the mixture.
Claims26
A method for selective enrichment of one or more RNA sequences in a complex sample comprising;
- a, reverse transcribing at least one RNA molecule to produce an RNA:cDNA hybrid molecule, using at least one reverse transcriptase and an exo-sample nucleotide that is incorporated into said cDNA;
- b, incubating the resulting cDNA:RNA hybrid with a source of Rnase H activity and an enzyme that specifically digests the cDNA sequence containing exo-sample nucleotide residues;
- thereby enriching one or more of said RNA sequences in the sample.
Dependencies are inferred from the claim text.