Methods and compositions for concentrating secreted recombinant proteins
AssigneeNew England Biolabs, Inc.
InventorsChristopher H. Taron, Paul A. Colussi
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Abstract
Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
Claims8
A preparation of Kluyveromyces cells characterized by a chitinase-negative phenotype wherein the phenotype is the result of a mutation in a chitinase gene expressing secreted chitinase, the preparation when grown for 48 hours being capable of producing substantially the same dry cell mass as wild-type Kluyveromyces cells.
Dependencies are inferred from the claim text.