Method of production of L-amino acids
AssigneeEvonik Degussa GmbH
InventorsBrigitte Bathe, Wilfried Claes
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Abstract
An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.
Claims23
A method for producing L-lysine comprising:
- a) fermenting a recombinant bacterium in a suitable nutrient medium to produce a fermentation broth containing biomass, wherein said recombinant bacterium comprises a polynucleotide that encodes a polypeptide possessing a citrate synthase activity, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 2, and (i) wherein L-aspartic acid at position 5 of the amino acid sequence is replaced by L-valine, and
- b) accumulating L-lysine in the nutrient medium, fermentation broth or in the cells of said bacterium,
- wherein the bacterium is Corynebacterium glutamicum.
Dependencies are inferred from the claim text.