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US 8,114,636 B2Granted
Labeling and detection of nucleic acids
AssigneeLife Technologies Corporation
InventorsBrian Agnew, Maura J. Ford, Kyle R. Gee, Kapil Kumar
Life of this patent
Estimating the term…
Abstract
Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.
Claims9
A method of forming a nucleic acid conjugate, wherein the method comprises:
- a) incorporating a terminal alkyne modified nucleotide into the nucleic acid polymer by contacting the terminal alkyne modified nucleotide with at least one other nucleotide in the presence of a DNA amplification enzyme to form a terminal alkyne modified nucleic acid polymer;
- and
- b) contacting the terminal alkyne modified nucleic acid polymer with a carrier molecule or solid support that comprises an azido moiety to form a nucleic acid polymer-carrier molecule, or nucleic acid polymer-solid support conjugate.
Dependencies are inferred from the claim text.