Sensing apparatus and method
AssigneeDNA Electronics Limited
InventorsChristofer Toumazou, Sunil Purushothaman
Life of this patent
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Abstract
A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
Claims31
A method of observing a chemical reaction comprising one or more nucleotide insertions at the end of a nucleotide chain in a DNA extension reaction, the method comprising:
- (a) detecting an electrical signal output from an ion sensitive field effect transistor (ISFET) exposed to said reaction, and
- (b) monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal consequent upon proton release with each nucleotide insertion to identify one or more individual chemical reaction events.
A method of observing a chemical reaction comprising addition of one or more nucleotide bases to a second polynucleotide to identify a portion of a polynucleotide template, the method comprising:
- (a) detecting an electrical signal output from an ion sensitive field effect transistor (ISFET) exposed to said polynucleotide template;
- (b) contacting the polynucleotide template with said second polynucleotide, serving as a primer or a probe, and with a solution of one or more different nucleotide bases;
- and
- (c) monitoring the detected electrical signal to discriminate fluctuations in the electrical signal indicating the release of protons due to the addition of one or more of said nucleotide bases to the second polynucleotide.
Dependencies are inferred from the claim text.