Modified oncolytic viruses
AssigneeViralytics Limited
InventorsEva Susanne Johansson, Darren Raymond Shafren
Life of this patent
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Abstract
An isolated selected Picornavirus capable of lytically infecting or killing a cell substantially in the absence of intercellular adhesion molecule-1 (ICAM-1).
Claims12
An isolated selected Coxsackie A21 virus capable of lytically infecting or inducing apoptosis in a cell substantially in the absence of intercellular adhesion molecule-1 (ICAM-1), wherein the selected virus comprises:
- (i) a capsid protein encoded by the nucleotide sequence of SEQ ID NO:
- 7, or
- (ii) a variant capsid protein encoded by a variant of the nucleotide sequence of SEQ ID NO:
- 7, the variant capsid protein comprising:
- (a) VP3 H96 or a conservative variation thereof wherein the conservative variation includes K, or
- (b) VP3 A101 or a conservative variation thereof wherein A101 is substituted by an amino acid selected from the group consisting of G, T, S, and M, or
- (c) both (a) and (b).
An isolated selected Coxsackie A21 virus in the form of CVA21-DAFv comprising a capsid protein encoded by the nucleotide sequence as set forth in SEQ ID NO:
- 7.
An isolated selected Coxsackie A21 virus capable of lytically infecting or inducing apoptosis in a cell substantially in the absence of intercellular adhesion molecule-1 (ICAM-1), wherein the selected virus comprises:
- (i) a capsid protein having the amino acid sequence as set forth in SEQ ID NO:
- 8, or
- (ii) a capsid protein that is a variant of the amino acid sequence set forth in SEQ ID NO:
- 8, the variant comprising:
- (a) a conservative substitution of VP3 H96 being VP3 H96K, or
- (b) a conservative substitution of VP3 A101 selected from the group consisting of VP3 A101G, VP3 A101T, VP3 A101S, and VP3 A101M, or
- (c) both (a) and (b).
Dependencies are inferred from the claim text.