PRRS viruses, infectious clones, mutants thereof, and methods of use
AssigneeRegents of the University of Minnesota
InventorsKay S. Faaberg, Jun Han, Gongping Liu, Yue Wang
Life of this patent
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Abstract
The present invention provides isolated infectious polynucleotides, such as infectious clones, having a nucleotide sequence with identity to PRRS viruses such as VR-2332, Lelystad, or others, and optionally further including a deletion in a region of ORF1 that encodes the nsp2 polypeptide.
Claims25
An isolated infectious polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and a deletion of at least 57 consecutive nucleotides corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14.
An isolated polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and at least one deletion of at least 57 consecutive nucleotides corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14, and wherein the polynucleotide replicates and produces infectious virus particles when introduced into a cell.
An isolated infectious polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:1 and a deletion of at least 39 consecutive nucleotides corresponding to nucleotide 2062 to nucleotide 3864 of SEQ ID NO:1, wherein the polynucleotide further comprises an exogenous polynucleotide present in the deletion.
An isolated polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 1 and at least one deletion of at least 39 consecutive nucleotides corresponding to nucleotide 2062 to nucleotide 3864 of SEQ ID NO:1, wherein the polynucleotide replicates and produces infectious virus particles when introduced into a cell, and wherein the polynucleotide further comprises an exogenous polynucleotide present in the deletion.
An isolated infectious polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and 2 or more deletions of at least 37 consecutive nucleotides each corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14.
An isolated infectious polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and a deletion of at least 39 consecutive nucleotides corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14, wherein the polynucleotide further comprises an exogenous polynucleotide present in the deletion.
An isolated polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and 2 or more deletions of at least 39 consecutive nucleotides each corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14, and wherein the polynucleotide replicates and produces infectious virus particles when introduced into a cell.
An isolated polynucleotide comprising a nucleotide sequence having at least 88% identity to SEQ ID NO:
- 14 and at least one deletion of at least 39 consecutive nucleotides corresponding to nucleotide 2061 to nucleotide 3545 of SEQ ID NO:
- 14, wherein the polynucleotide replicates and produces infectious virus particles when introduced into a cell, and wherein the polynucleotide further comprises an exogenous polynucleotide present in the deletion.
An isolated infectious polynucleotide comprising a nucleotide sequence SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
An nsp2 polypeptide encoded by an infectious polynucleotide comprising a nucleotide sequence SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
Dependencies are inferred from the claim text.