Microfluidic cartridge
AssigneeHandyLab, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.
Claims36
A microfluidic substrate, comprising:
- a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another:
- an inlet;
- a first valve and a second valve;
- a reaction chamber;
- a vent;
- a first channel leading from the inlet, via the first valve, to the reaction chamber;
- and
- a second channel leading from the reaction chamber, via the second valve, to the vent;
- wherein the first and second valves are co-planar with the first and second channels in the microfluidic network and wherein the first and second valves each comprise a temperature responsive substance that melts upon heating and seals the first and second channels.
A microfluidic cartridge, comprising:
- a substrate having an upper side and an opposed lower side, wherein the substrate comprises a plurality of sample lanes, wherein each lane of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another:
- an inlet;
- a first valve and a second valve;
- a first channel leading from the inlet, via the first valve, to a reaction chamber;
- a second channel leading from the reaction chamber, via the second valve, to a vent;
- and
- a label, attached to the upper side, wherein each valve has a wax loading hole extending to the upper side of the substrate, and wherein the label covers and seals the loading holes.
A microfluidic substrate, comprising:
- a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another:
- an inlet;
- a first valve and a second valve;
- a reaction chamber;
- a vent;
- a first channel leading from the inlet, via the first valve, to the reaction chamber;
- and
- a second channel leading from the reaction chamber, via the second valve, to the vent;
- wherein the first and second valves comprise a temperature responsive substance that melts upon heating and seals the reaction chamber.
Dependencies are inferred from the claim text.