Methods for detecting vitamin D metabolites by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsNigel Clarke, Brett Holmquist, Kwang-Ja Lee, Richard E. Reitz
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Abstract
Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.
Claims23
A method for determining the amount of a vitamin D metabolite in a sample by tandem mass spectrometry, comprising:
- (a) generating a protonated and dehydrated precursor ion of said vitamin D metabolite;
- (b) generating one or more fragment ions of said precursor ion;
- and
- (c) detecting the amount of one or more of said ions generated in step (a) or (b) or both and relating the detected ions to the amount of said vitamin D metabolite in said sample.
A method for determining the amounts of two or more vitamin D metabolites in a sample in a single assay, said method comprising:
- (a) ionizing two or more vitamin D metabolites to generate protonated and dehydrated precursor ions specific for each of said two or more vitamin D metabolites;
- (b) generating one or more fragment ions of each of said precursor ions;
- and
- (c) detecting the amount of one or more of said ions generated in step (a) or (b) or both and relating the detected ions to the amounts of said two or more vitamin D metabolites in said sample.
Dependencies are inferred from the claim text.