US 8,093,451 B2 · Chalcone synthase dihydroflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue

Abstract

The present invention relates to nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, flavonoid biosynthesis in plants, and more specifically the modification of the content of condensed tannins. In particularly preferred embodiments, the invention relates to the combinatorial expression of chalcone synthase (CHS) and/or dihydroflavonol 4-reductase (BAN) and/or leucoanthocyanidine reductase (LAR) in plants to modify, for example, flavonoid biosynthesis or more specifically the content of condensed tannins.

Claims16

Dependent claims

A nucleic acid construct comprising

a first nucleic acid portion encoding a chalcone synthase (CHS), said first nucleic acid portion comprising a nucleotide sequence selected from the group consisting of:
(a1) Sequence ID No. 1;
and
(b1) nucleotide sequences encoding Sequence ID No. 2;
and
a second nucleic acid portion encoding anthocyanine reductase, said second nucleic acid portion comprising a nucleotide sequence selected from the group consisting of:
(a2) Sequence ID No. 9;
and
(b2) nucleotide sequences encoding Sequence ID No. 10,
said construct increasing the levels of both CHS and anthocyanine reductase when the construct is introduced into a plant cell.

7 more elements

A nucleic acid construct comprising

a first nucleic acid portion for reducing the amount of chalcone synthase (CHS), said first nucleic acid portion comprising a nucleotide sequence selected from the group consisting of:
(A1) Sequence ID No. 1, and the complements thereof;
(B1) nucleotide sequences encoding Sequence ID No. 2, and the complements thereof;
and
(C1) functionally active fragments of the sequences recited in (A1), and (B1), said functionally active fragments having a size of at least 60 nucleotides;
and
a second nucleic acid portion for reducing anthocyanine reductase, said second nucleic acid portion comprising a nucleotide sequence selected from the group consisting of:
(A2) Sequence ID No. 9 and the complement thereof;
(B2) nucleotide sequences encoding Sequence ID No. 10 and the complement thereof;
and
(C2) functionally active fragments of the sequences recited in (A12) and (B2), said functionally active fragments having a size of at least 60 nucleotides;
said construct reducing the levels of both CHS and anthocyanine reductase when the construct is introduced into a plant cell.

9 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/552,857Filed 14 Apr 2004

Priority

AU 200390179714 Apr 2003

Published

US 2006/0195920 A131 Aug 2006

Granted

10 Jan 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 9/0093
Oxidoreductases: acting on CH or CH2 groups
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 9/1037
Transferring groups other than amino-acyl groups: naringenin-chalcone synthase , i.e. chalcone synthase

Chalcone synthase dihydroflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue

Life of this patent

Chalcone synthase dihydroflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue

Life of this patent

Abstract

Claims16