Fed-batch cell culture methods using non-animal-based hydrolysates
AssigneeAbbott Laboratories
InventorsItzcoatl A. Pla, Joseph G. Matuck, John C. Fann, Christof Schulz, Nichole A. Roy, David F. Bruton, James McIntire, David Chang Yu-Hsiang, Thomas Seewoester
Life of this patent
Estimating the term…
Abstract
The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
Claims65
A fed batch method of producing a protein comprising
- a) culturing mammalian cells comprising a nucleic acid encoding the protein in a cell culture comprising a cell culture production medium;
- and
- b) feeding the mammalian cells by adding a hydrolysate enrichment solution and a basal enrichment solution to the cell culture during a time period,
- wherein the hydrolysate enrichment solution comprises a plant-based hydrolysate and 75-300 g/L of a yeast-based hydrolysate,
- such that the protein is produced.
A fed batch method of producing an anti-TNFα antibody, or a fragment thereof, comprising
- a) culturing Chinese Hamster Ovary (CHO) cells comprising a nucleic acid encoding the anti-TNFα antibody, or fragment thereof, in a cell culture comprising a cell culture production medium;
- and
- b) feeding the CHO cells by adding a hydrolysate enrichment solution and a basal enrichment solution to the cell culture during a time period,
- wherein the basal enrichment solution comprises a plant-based hydrolysate and 75-300 g/L of a yeast-based hydrolysate such that the anti-TNFα antibody, or fragment thereof, is produced.
A fed batch method of producing an anti-IL-12 antibody, or a fragment thereof, comprising
- a) culturing CHO cells comprising a nucleic acid encoding the antibody in a cell culture comprising a cell culture production medium,
- b) feeding the CHO cells by adding a hydrolysate enrichment solution and a basal enrichment solution to the cell culture during a time period,
- wherein the basal enrichment solution comprises a basal medium, asparagine, and glucose, and
- wherein the hydrolysate enrichment solution comprises a plant-based hydrolysate and 75-300 g/L of a yeast-based hydrolysate such that the anti-IL-12 antibody, or fragment thereof, is produced.
A fed batch method of producing adalimumab, or a fragment thereof, comprising
- a) culturing mammalian cells expressing adalimumab, or a fragment thereof, in a cell culture, wherein the mammalian cells are cultured at a constant temperature between 32-38° C.;
- and
- b) feeding the mammalian cells by adding a hydrolysate enrichment solution and a basal enrichment solution to the cell culture, wherein the hydrolysate enrichment solution comprises a plant-based hydrolysate and 75-300 g/L of a hydrolysate which is not derived from a plant or an animal, such that adalimumab, or fragment thererof, is produced.
Dependencies are inferred from the claim text.